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The University of Tennessee

The College of Veterinary Medicine

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Faculty

David A. Brian, D.V.M., Ph.D.,
Professor
Department of Pathobiology
College of Veterinary Medicine
The University of Tennessee
2407 River Drive
Knoxville, TN 37996-4542
Phone: (865) 974- 4030
FAX: (865) 974-5616
Email
URL
http://web.bio.utk.edu/micro/faculty/brian.html

Education

  • B.A., Biology, Manchester College, North Manchester, Indiana, 1964
  • D.V.M. (with honor), Michigan State University, College of Veterinary Medicine, 1969
  • Ph.D., Molecular Virology, Department of Microbiology and Public Health, Michigan State University, 1974

Teaching

  • Virology

Research Interests

  • Mechanisms or RNA replication and regulation of gene expression from RNA virus genomes coronaviruses.

Professional Societies

  • American Society for Microbiology
  • American Society for Virology
  • Member, Coronaviridae Subcommittee, International Committee on Taxonomy of Viruses: First term, 1988-1993; Second term, 1993-2000; Third term, 2001-2006.

Publications

  • Sethna, Phiroze B., Shan-Ling Hung, and David A. Brian. 1989. Coronavirus subgenomic minus-strand RNA and the potential for mRNAreplicons. Proc. Natl. Acad. Sci., U.S.A. 86:5626-5630.
  • Kienzle, Thomas E., Sushma Abraham, Brenda G. Hogue, and David A. Brian. 1990. Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein. J. Virol. 64:1834-1838.
  • Hofmann, Martin A., Phiroze B. Sethna, and David A. Brian. 1990. Bovine coronavirus mRNA replication continues throughout persistent infection in cell culture. J. Virology 64:4108-4114.
  • Sethna, Phiroze B., Martin A. Hofmann, and David A. Brian. 1991. Minus-strand copies of replicating coronavirus mRNAs contain antileaders. J.Virol. 65:320-325.
  • Hofmann, Martin A., andDavid A. Brian. 1991. The 5-prime end of coronavirus minus-stand RNAs contain a short poly(U) tract. J. Virol.65:6331-6333.
  • Senanayake, Savithra D., Martin A. Hofmann, Joanne L. Maki, and David A. Brian. 1992. The nucleocapsid protein gene of the bovine coronavirus is bicistronic. J. Virol. 66:5277-5283.
  • Hofmann, Martin A., Ruey-Yi Chang, Seulah Ku, and David A. Brian. 1993. Leader-mRNA junction sequences are unique for each subgenomic mRNA species in the bovine coronavirus and remain so throughout persistent infection. Virology, 196:163-171.
  • Hofmann, Martin A., Savithra D. Senanayake, and David A. Brian. 1993. A translation-attenuating intraleader open reading frame is edon coronavirus mRNAs during persistent infection. Proc. Natl. Acad.Sci. USA, 90:11733-11737.
  • Chang, Ruey-Yi, MartinA. Hofmann, Phiroze B. Sethna, and David A. Brian. 1994. Acis-acting function for the coronavirus leader in defective-interfering RNA replication. J. Virol. 68:8223-8231.
  • Brian, David A., Thomas E. Kienzle, and Brenda G. Hogue. 1995. The hemagglutinin-esteraseprotein. In: The Coronaviridae. S. Siddell and V. ter Meulen (eds). Plenum Press. pp. 165-176.
  • Chang, Ruey-Yi, andDavid A. Brian. 1996. Cis-requirement for N-specific protein sequence in bovine coronavirus defective interfering RNA replication. J. Virol. 70:2201-2207.
  • Chang, Ruey-Yi, RajeshKrishnan, and David A. Brian. 1996. The UCUAAAC promoter motif is not required for high frequency leader recombination in bovine coronavirus defective interfering RNA. J. Virol. 70:2720-2729.
  • Krishnan, Rajesh, Ruey-Yi Chang, and David A. Brian. 1996. Tandem placement of a coronavirus promoter results in enhanced mRNA synthesis from the downstream-most initiation site. Virology. 218:400-405.
  • Senanayake, Savithra D., and David A. Brian. 1997. Bovine coronavirus I protein synthesis follows ribosomal scanning on the bicistronic N mRNA. Virus Research 48:101-105.
  • Sethna, Phiroze B., andDavid A. Brian. 1997. Coronavirus subgenomic and genomic minus-strand RNAs are found in N protein-deficient, membrane-protected replication complexes. J. Virol.71:7744-7749.
  • Brian, David A., and Willy Spaan. 1997. Recombination and coronavirus defective interfering RNAs. Seminars in Virology 8:101-111.
  • O' Connor, Jennifer B., and David A. Brian. 1999. The major product of procine transmissible gastroenteritis coronavirus gene 3b is an integral membrane glycoprotein of 31 kDa. Virology 256:152-161.
  • Senanayake, Savithra D., and David A. Brian. 1999. Translation from the 5' UTR of mRNA 1 is repressed, but that from the 5' UTR of mRNA 7 is stimulated in coronavirus infected cells. J. Virol. 73:8003-8009.
  • Williams, Gwyn D., Ruey-Yi Chang, and David A. Brian. 1999. A phylogenetically conserved hairpin-type 3' UTR pseudoknot functions in coronavirus RNA replication. J. Virol. 73:8349-8355.
  • O' Connor, Jennifer B., and David A. Brian. 2000. Downstream ribosomal entry for translation of coronavirus TGEV gene 3b. Virology 269:172-182.
  • Brian, David A. 2001. Nidovirus genome replication and subgenomic mRNA synthesis: Pathways followed and cis-acting sequences required. A review in Nidoviruses, Plenum Press. In press.
  • Ozdarendeli, Aykut., Seulah Ku, Sylvie Rochat, Gwyn D. Williams, Savithra D. Senanayake, and David A. Brian. Downstream sequences influence the choice between a naturally-occurring noncanonical and closely-positioned upstream canonical promoter during bovine coronavirus subgenomic mRNA synthesis. Submitted to J. Virol.