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The University of Tennessee

The College of Veterinary Medicine

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Barry T. Rouse, BVSc, Ph.D., DSc


Lindsay Young Distinguished Professor of Microbiology
Department of Pathobiology
College of Veterinary Medicine
The University of Tennessee
Walter's Life Science Building, F403
Knoxville, TN 37996
Phone: (865) 974-4026
FAX: (865) 974-4007
Email
http://web.bio.utk.edu/micro/faculty/rouse.html

Education

  • University of Bristol - 1965 - Bachelor of Veterinary Science with honors
  • University of Guelph - 1967 - M.Sc.
  • University of Guelph - 1970 - Ph.D.
  • Walter and Eliza Hall Institute of Medical Research - 1970-72
  • University of Bristol - 1997 - D.Sc.

Teaching

  • Immunology

Funding

  • Three RO1 grants from National Institutes of Health

Research Interests

  • Immunobiology and immunopathology of virus infections. Current Projects: Our research uses various animal models of Herpes simplex virus (HSV) infections in an attempt to understand the immunopathogenesis of HSV infection and its control by vaccines. In one model, herpetic stromalkeratitis (HSK) lesions are immunopathological. We are attempting to understandthe molecular and cellular mechanisms involved in the pathogenesis of HSK with a view to defining means of controlling these lesions which are an important cause of human blindness. The disease process appears to result from a pathological immune reaction against viral proteinsor host proteins induced following infection or by a bystander mechanism of activation. The mechanism of immunopathology as well as its modulation is under investigation. Other projects deal with defining optimal means of inducing protective immunity against HSV. Current research emphasizes DNA vaccines given via mucosal routes. A range of in vivo and in vitro test systems are used to evaluate efficacy. In addition, we are attempting to define the relevance of cross-priming during immunization against viruses and determining how the cross priming process is mediated in vitro and in vivo.

Professional Activities

  • Section Editor, Journal of Immunology
  • Member, Editorial Board of Virology, Viral Immunology, Journal of Virology
  • Principal Organizer, Keystone Symposium on Molecular Aspects of Viral Immunity, 2001
  • Alcon Award for Vision Research, 2000

Publications

  • Thomas, J., and B. T. Rouse. 1998. Immunopathology of herpetic stromal keratitis: discordance in CD4+ T cell function between euthymic host and reconstituted SCID recipients. J.Immunol. 160:3965-3970.
  • Gangappa, S. P., E. Manickan, and B. T. Rouse. 1998. Control of herpetic stromal keratitis by CTLA-Ig. Clin & Exp. Immunol. Immunopathol. 86:88-94.
  • Kuklin, N. A., M. Daheshia, P. C. Marconi, D. M. Krisky, R. J.D. Rouse, J. C. Glorioso, E. Manickan, and B. T. Rouse. 1998. Modulation of mucosal and systemic immunity by enteric administration of nonreplicating herpes simplex virus expressing cytokine genes. Virology. 240:245-253.
  • Kumaraguru, U., and B. T. Rouse, 1998. DNA vaccines for prophylaxis and modulation of HSV infections. Exp. Opin. In Invest. Drugs 7:1-6.
  • Rouse, B. T., S. Nair, R. J. D. Rouse, Z. Yu, N. Kuklin, K. Karem, and E. Manickan. 1998. DNA vaccines and immunity to herpes simplex virus. Current Topics Microbiol. Immunol. 226:69-78.
  • Daheshia, M., N. A. Kuklin, E. Manickan, S. Chun, and B.T. Rouse. 1998. Immune induction and modulation by topical ocular administration of plasmid DNA encoding antigens and cytokines. Vaccine. 16:1103-1110.
  • Yu, Z., K. Karem, S. Kanangat, E. Manickan, and B.T. Rouse. 1998. Protection by minigene: novel approach of DNA vaccination. Vaccine. 16:1660-1667.
  • Kumaraguru, Y., I. Davis, and B.T. Rouse. 1998. Chemokines andocular pathology caused by corneal infection with herpes simplexvirus. J. Neurovirol. 4:1-6.
  • Kuklin, N. A., M. Daheshia, S. Chun, and B.T. Rouse. 1998. Role of mucosal immunity in herpes simplex virus infection. J. Immunol. 160:5998-6003.
  • Thomas, J., S.P. Gangappa, S. Chun, M. Daheshia, and B.T.Rouse. 1998. Immunopathogenesis of herpetic stromal keratitis, a blinding disease of mankind. Central Europ. J. Immunol. 23:72-83.
  • Thomas, T. J., S. Kanangat, and B.T. Rouse. 1998. Herpes simplex replication induces expression of chemokines and proinflammatory cytokines in eye implications in herpetic stromal keratitis. J. Interferon. & Cytokine Res. 18:681-690.
  • Rouse, B. T., and N. A. Kuklin. 1998. Respiratory viral vaccines. In Mucosal Immunology, 2nd Edition, Edited by P.L. Ograet al., Academic Press, NY. 895-901.
  • Manickan, E., M. Daheshia, N. Kuklin, S. Chun, and B.T. Rouse. 1998. Modulation of viral induced delayed type hypersensitivity by plasmid DNA encoding the cytokine IL-10. Immunology. 94:129-134.
  • Gangappa, S. P., J. S. Babu, S. Kanangat, J. Thomas, Daheshia, M., and B. T. Rouse. 1998. Virus induced immuno inflammatorylesions in the absence of viral antigen recognition. J. Immunol. 161:4289-4300.
  • Kuklin, N., M. Daheshia, S. Chun, and B. T. Rouse. 1998. Immunomodulation by mucosal gene transfer using TGFbeta DNA. J.Clin. Invest. 102:438-444.
  • Chun, S., M. Daheshia, N. A. Kuklin, and B. T. Rouse.1998. Modulation of viral immunoinflammatory responses with cytokine DNA administered by different routes. J. Virol. 72:5351-5359.
  • Davis, I. A., and B. T. Rouse. 1998. Immuneresponsiveness of lymphotoxin-alpha deficient mice: two reconstitution models. Cell. Immunol. 189:116-124.
  • Davis, I. A., and B. T. Rouse. 1998. The spleen and organized lymph nodes are not essential for the development of gut-induced mucosal immune responses in lymphotoxin-deficient mice. Clin. Immunol. & Immunopathology. 89:150-159.
  • Rouse, B. T., and S. B. Atherton. 1998. Immunopathology of Herpesvirus Infections. In Infectious Agents and Pathogenesis. Edited by H. Friedman, P. Medveczky, and M. Bendinelli. Plenum Press, NY. pp. 33-51.
  • Daheshia, M., Feldman, L. T., and Rouse, B. T. 1998. Herpes simplex virus latency and the immune response. Current Opin. Immunol. 1:430-435.
  • Daheshia, M., S. Kanangat, and B. T. Rouse. 1998. Production of key molecules by ocular neutrophils early after corneal infection by herpes simplex virus infection - relevance to the pathogenesis of stromal keratitis. Exp. Eye Res. 67:619-624.
  • Rouse, B. T., and S. Kanangat. 1999. Persistent infection with herpes simplex virus. In Persistent Virus Infections. R. Ahmed and I. Chen, eds. John Wiley & Sons, pp.165-182.
  • Daheshia, M., N. A. Kuklin, S. Chun, S. Deshpande, and B. T. Rouse. 1999. Resistance to herpetic stromal keratitis in immune B cell deficient mice. Virology. 257:168-176.
  • Chun, S., M. Daheshia, S. J. Lee, and B. T. Rouse. 1999. Immune modulation of IL-10 gene transfer via viral vector and plasmid DNA: Implications for gene therapy. Cellular Immunology. 194:194-204.
  • Rouse, B. T. 1997. DNA Vaccines: Their use for prevention and modulation of herpes simplex virus infection. In: Genetic Vaccines and Immunotherapeutic Strategies, C. Thibeault and L. Savage, eds., International Business Communications, Inc. Southborough, MA pp. 145-148.
  • Chun, S., M. Daheshia, S. Lee, S. K. Eo, and B. T. Rouse. 1999. Distribution fate and mechanism of immune modulation following mucosal delivery of plasmid DNA encoding IL-10. J. Immunol. 163:2393-2402.
  • Kuklin, N., M. Daheshia, and B. T. Rouse. 1999. Mucosal immune induction by naked DNA administered intranasally. In Proceedings of 9th Int. Cong, in Mucosal Immunology.
  • Gangappa, S., S. Deshpande, and B. T. Rouse. 1999. Bystander activation of CD4+ T cells can represent an exclusive means of immunopathology in a virus infection. Europ. J. Immunol. 29:3674-3682.
  • Rouse, B. T., Daheshia, M., and D. S. Schmid. 2000. Herpes simplex virus and the immune response: A balance of power. In Effects of Microbes on the Immune System. Ed. By M. W. Cunningham and R. S. Fujinami. Lippencott-Raven, Philadelphia. pp.387-398.
  • Davis, I. A., and B. T. Rouse. 2000. Identification of lymphocyte homing molecules and vascular addressins in lymphotoxin-alpha deficient mice. Lab Animal. 34:111-114.
  • Gangappa., S., S. P. Deshpande, and B. T. Rouse. 2000. Bystander activation of CD4+ T cells accounts for herpetic ocular lesions. Invest. Ophthalmol. Vis. Sci. 41:453-459.
  • Deshpande, S. P., M. Zheng, M. Daheshia, and B. T. Rouse. 2000. Pathogenesis of herpes simplex virus induced ocular immuno inflammatory lesions in B cell deficient mice. J.Virology. 74:3517-3524.
  • Kumaraguru, U., R. J. D. Rouse, S. K. Nair, B. D. Bruce, and B. T. Rouse. 2000. Involvement of an ATP dependent peptide-chaperone in cross presentation after DNA immunization. J.Immunol. 65:750-759.
  • Deshpande, S. P., U. Kumaraguru, and B. T. Rouse. 2000. Dual role of B cells in mediating innate and acquired immunity to herpes simplex virus infection. Cell Immunol. 207: 79-87.
  • Cormack, B., B. T. Rouse, and S. M. Beverly. 1999. Host-microbe interactions in fungi, viruses, and parasites. Editorial overview. Curr. Opin. Microbiol. 2:343-347.
  • Deshpande, S., U. Kumaraguru, and B. T. Rouse. 2000. Why do we lack an effective vaccine against HSV infections? Microbes & Infection. 2:1-6.
  • Kumaraguru, U. and B. T. Rouse. 2000. Application of the intracellular interferon-assay to recalculate the potency of CD8+ Tcell responses to HSV. J. Virol. 74:5709-5711.
  • Lee, S., S. K. Eo, and B. T. Rouse. 2000. Immunization via the gastric mucosa -- an approach to boost and modulate immunity with DNA vaccine. Central European J. Immunol. 25: 130-136.